Oct 2 2007
A note from Pam Owens at TriCore Reference Laboratories:
We have seen a reference to a Factor 13 assay using thrombin and acetic acid as opposed to 5M urea.
Does anyone have experience with this method? Where can we get a copy of the protocol?
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Dear Pam, we did use this method: in glass tube 0.1 mL plasm
Dear Pam, we did use this method: in glass tube 0.1 mL plasma, 0.1 mL saline, warm to 37C, clot with 0.1 mL CaCL, leave for 10 min 37C to stabilize clot. Then add 0.6 mL 2% acetic acid, leave on bench 2 hrs then observe for clot dissolution. Sensitive to 2% FXIII. Do not shake at any time as shaking causes clot retraction. Positive control use EDTA plasma. Have now changed to the IL method, but use this as confirmatory test when IL result is <5%. Robyn Coleman