On March 6 I posted comments from the Precision BioLogic, Inc. technical staff onspecimen freezing. CEO Steve Duff and I both commented on the dearth of data that may support freezing temperature choices. Here is another reference on the subject, McGlasson D. Standardization of temperature in handling and storage of plasma for coagulation testing. Texas J Med Technol 1985; 2: 10-11. The article indicates that factor VIII levels become significantly reduced with stored at –20°C when compared to fresh specimens and those stored at –70°. To see the paper, click on McGlasson Storage Temperature 1985.