From Heather DeVries. Hi George! Do you know the reasoning behind using a 2-hour vs 1-hour incubation in mixing studies? We have always used 2 hours, and I wonder if it has to do with the heterogeneity of the antibodies we are trying to pick up. Our pathologist probably has a very good explanation, but I thought it would be interesting to hear from others on what they do and why. Thanks, Heather.
Hi, Heather, I have ordered a 2016 and a 2017 article on mixing studies, may have more to add tomorrow, but for now, the incubation period seems to be simply a matter of local preference. I once asked someone in the UAB special coagulation lab how long he incubated, his response was, “depends on how close we are to the end of the shift.” I’ve not seen any data that validate one interval over another, and I don’t know of any IgG immunoglobulins that require 2 hours to react. More on Wednesday, I hope.