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Follow-up to Incubated Mixing Studies

Referring to Ruth Spates’ June 18, 2018 question about mixing studies followed by Dr. Paul Riley’s comment asking why we incubate two hours, Dr. Connie Miller of the CDC answered today with reference to a 1974 publication that establishes hemophilia A inhibitor (anti-FVIII) kinetics. Click or tap here for the full 1974 article, Allain JP, Frommel D, Antibodies to factor VIII: specificity and kinetics of iso- and hetero-antibodies in hemophilia A. Blood 1974; 44: 313–22. It looks like two hours remains the optimum time.

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