Dear George, One day old baby citrate sample, unable to separate plasma after centrifugation. Repeated with fresh sample, same problem. What is the cause? With regards, Vilas Hiremath, United Labs.
Hello, Vilas, it is always good to hear from you. Does this mean no plasma layer appears after centrifugation? If so, perhaps polycythemia has made the hematocrit exceptionally high, leaving very little plasma layer, or there is considerable hemolysis so the plasma layer is difficult to distinguish.This could also mean the plasma is clotted (serum) and therefore difficult to extract. Ruling out a collection error, it could mean there is chronic DIC causing excessive clotting. I’ve emailed this to several colleagues to take advantage of their experience, so watch this post for comments. Geo.