Jan 27 2014
Each year George enjoys the opportunity to teach in Dr. Elaine Keohane’s graduate course, CLSC 5124, Advanced Hemostasis at Rutgers University School of Health Related Professions. Our students are experienced medical laboratory scientists from all over the world, and they bring a wealth of practical knowledge. Dr. Keohane is teaching a section on platelet physiology this week, and the question came up, what is platelet factor 3? I’m old enough to know the answer, but I’d like to hear from our participants. In addition to defining PF3, also please report what coagulation laboratory assay we used to “diagnose” PF3 deficiency.
Comments (2)
Bleeding Disorders
Hello, Ali. The test was actually called the “serum prothrom
Hello, Ali. The test was actually called the “serum prothrombin time” or the “prothrombin consumption test.” Draw whole blood into glass and allow to clot for exactly 2 hours, then perform a standard PT. If it clots in less than 25 seconds, you presume there is a deficiency of PF3. Many pitfalls, of course!
Platelet Factor-3 was an old name for lipid or lipoprotein d
Platelet Factor-3 was an old name for lipid or lipoprotein derived from platelet that accelerate coagulation cascade process. At that time, purification was not great so composition at best was known as phospholipids. Recently they have been characterised as predominantly phosphatidyl serine, ethanolamine and inositol. George, I am not old enough to remember the name of test, but I am guessing platelet rich plasma after stimulation of platelet with an activator does the job. What was the name of assay anyway?