Dear George, For heparin-induced thrombocytopenia (HIT) serotonin release assay, can serotonin ELISA sensitive to HIT be used? With regards, Vilas Hiremath.
Hello, Vilas, always good to hear from you. I had to go to experts, Drs Larry Brace, Edward Hospital in Naperville IL, and Jon Geske, Precision BioLogic Manager of Emerging Technologies & Markets. Here is a reply from Dr. Geske:
Theoretically, a serotonin ELISA could be used to measure serotonin released from platelets activated by antibodies from HIT patients. Unlike the gold standard 14C-serotonin release assay, however, one would have to differentiate between serotonin present in the patient’s sample vs. serotonin released from the activated platelets. Controls would have to be run (low vs. excess heparin levels) similar to the 14C assay to detect PF4/heparin antibody-dependent platelet activation. Furthermore, the ELISA method for serotonin quantification is complicated by the fact that many such assays require serotonin acylation prior to running the ELISA. Having cleared those hurdles, however, an ELISA could be used to detect serotonin levels. I hope this helps. Jon.