From Vanessa Chan at Sick Kids in Toronto: Hi George and the coag world, what assay is everyone using to monitor unfractionated heparin (UFH) and low molecular weight heparin (LMWH)? What are everyone’s thoughts on the use of antithrombin and/or dextran sulfate in these reagents? In your external quality assessments (EQAs), do you experience any biased results with your assays? Stago is replacing their AT-DS-Rotachrom reagent with Liquid Anti-Xa, I’m wondering if there are other AT-DS reagents available and what other labs suggest?
Thank you for the information, Vanessa. Participants, please provide your information.
Very nice point about DS and exogenous AT in anti-Xa assay i
Very nice point about DS and exogenous AT in anti-Xa assay is stated in recent review:
“Thus, assays that add exogenous AT or dextran sulfate may overestimate the actual in vivo activity of UFH, LMWH, or fondaparinux in patients with excess plasma proteins or deficient levels of AT.”
Test of the Month: The chromogenic antifactor Xa assay
Eric Gehrie and Michael Laposata