I spoke with Patti Tichenor, UAB special coagulation laboratory who had discussed our December 5, 2012 factor VIII linearity question from Mahnaz Sairi with her UAB colleague Laura Taylor. UAB uses high-end coagulometers (Stago STA-R) for their factor assays, and their linearity extends from near zero to 100%. When they anticipate especially high factor VIII levels, they will order dilutions to as high as 1:60 (recall the initial dilution is 1:10). Using this method, they recently report a FVIII activity level of over 700% on a renal failure patient.
Jan 11 2013
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Factor Assays
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