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Hybrid Curve Controls for Anti-Xa Heparin Assay

This is from frequent contributor Crystal AzevedoEastern Maine Healthcare Systems. Hi George, Look forward to seeing you next month in Portland, Maine! I am setting up a hybrid curve for heparin monitoring using the Rotachrom Heparin reagent and Stago’s hybrid calibrator. Which control material should be used with this curve? Thanks!

Hi, Crystal, I look forward to seeing you also, and thanks for the plug. I will be giving two presentations at the Northeast Laboratory Conference at the Holiday Inn in Portland, Maine on Tuesday, October 16, and Wednesday, October 17, 2012, sponsored by Stago, represented by account exec Dale Harrison. I turned once again to hybrid curve specialist Dave McGlasson for help with your question, and here is his reply:

Stago now has a new liquid heparin assay with three calibrators that include unfractionated heparin (UFH) and low molecular weight heparin (LMWH). I used for the STA-Rotachrom anti-Xa assay the following calibrators from Stago: for the UFH I used theSTA-Hepanorm H calibrators (product #00683) 0, 3 and 6 with UFH concentrations 0.0, 0.33, 0.49 IU/mL. The LMWH used the HBPM/LMWH calibrators (product # 00686) 0, 9, 18 (LMWH concentrations 0.0, 0.86, 1.85 IU/mL). I would advise to contact the vendor for current information. See also McGlasson DL. Using a single calibration curve with the anti-Xa chromogenic assay for monitoring heparin anticoagulation. Lab Medicine 2005;36:297–9. The article is posted below, and by the way, Dave is also speaking in Portland, on Wednesday, October 17.

McGlasson Lab Medicine Article 2005

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