Jul 21 2013
From Saravanan Vinayagam: We are trying to set up the factor VIII inhibitor assay on our ACL TOP analysers. Has anyone got an SOP or previous experience in validating inhibitor assay in an automated analysers? Thank you.
Hello, Saravanan Vinayagam, and thank you for your post. I’m hoping that an IL technical representative will see this and offer some assistance. Geo.
Comments (3)
Bleeding Disorders
Note: We had previously validated our Factor VIII assay on t
Note: We had previously validated our Factor VIII assay on the TOP. Since the only part of the Bethesda assay performed on the TOP is the factor VIII assay, we only needed to run a known sample to prove recovery. I hope this addresses your question? We have both a validation procedure for automated analyzers and a Bethesda assay procedure if either of these would help.
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Do you have a procedure for the Bethesda Assay? We perform a
Do you have a procedure for the Bethesda Assay? We perform all of the dilutions and mixes manually for our Bethsda assay, incubate in a 37 degrees water bath for two hours, then run all of the dilutions on the TOP for a factor VIII (or whatever factor you are looking for the inhibitor) on the X10 dilution only, not the parallelism set-up. Your IL rep can help you set up a separate test for just the X10 dilution for this purpose. Then use the recovered values to calculate your Bethesda units. We already had the Bethesday assay procedure when we went live with the TOP so we just had to set-up the test for the X10 (straight) factor VIII, and then run a known Bethesda sample and make sure we recovered the correct value. We do very well using this method on our survey samples.
In the simplist terms the Factor VIII inhibitor assay is not
In the simplist terms the Factor VIII inhibitor assay is nothing more than a Factor VIII assay performed on serial dilutions on a test plasma. No special test setup is required on the instrument.
Regards,
Herb Crown
St. Louis University Hospital Coagulation Reference Lab