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Elagic Acid-based PTT Reagent

From Kelly Rose at Novartis: Hi George, I work in a research lab and we are interested in comparing different partial thromboplastin time (PTT) reagents on our Stago Sta-R. Currently we use the silica-based (STA PTT-Automate) and kaolin-based STA C. K. Prest. We would like to try ellagic acid. Have you had any experience with this reagent? and if so what kit would you recommend?

Hi, Kelly, and thank you for your question. I recommend you use either IL SynthAFax or Dade Actin FS, distributed by Siemens. Sorry, I’ve not had direct experience with either reagent, but both can be adapted to the Stago instrument. For additional information, please check our recent article, Fritsma GA, Dembitzer FR, Randhawa A, Marques MB, Van Cott EM, Adcock-Funk D, Peerschke EI. Recommendations for appropriate activated partial thromboplastin time reagent selection and utilization. Am J Clin Pathol 2012;137: 904–8.

Comments (2)
Screening Assays
Oct 9, 2012 11:22am

Hi Kelly, we use Actin FS on our STA-R but it tarnishes need
Hi Kelly, we use Actin FS on our STA-R but it tarnishes needle #2 which means we need to change that needle every PM.

Sep 27, 2012 4:01am

Depends on whether you want an LA ‘sensitive’ or ‘insensitiv
Depends on whether you want an LA ‘sensitive’ or ‘insensitive’ reagent. Actin FS is fairly insensitive, but Actin FSL is fairly sensitive; for more info, refer to: Kershaw G, Suresh S, Orellana D, Nguy YM. Laboratory identification of lupus anticoagulants. Semin Thromb Hemost. 2012;38:375-84.

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