PT/INR Results Using ISI 1.0 Reagents

From Terezinha Paz Munhoz: we started to use recombinant thromboplastin,RecombiPlasTin 2G (ISI 1.0) on our IL ACL TOP 500. The PT results are quite different from rabbit brain reagent. The high sensitivity shows a wide range: 8–220 seconds. We know the method linearity in this equipment is 8,0–320 seconds, and the percentage activity (that we still report) is “crazy” […]

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Anticoagulant Therapy

Aug 27 2012

george

New QQ: History

Our August and September Quick Question, posted Monday, August 27, is mostly for fun, and will appeal most to our more “seasoned” coagulationists. No wrong answer, just some more right than others! Please indicate the assay most often used before the partial thromboplastin time (PTT) for monitoring heparin.

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Quick Question Archive

Aug 27 2012

george

Quick Question Summary: VWF Activity

Here is a summary of our July, 2012 Quick Question: How do you assay for von Willebrand factor (VWF) activity? a. VWF ristocetin cofactor (VWF:RCo) by aggregometry: 21 (34%) b. Automated VWF ristocetin cofactor (VWFRCo): 21 (34%) c. VWF activity immunoassay (VWF:Act): 15 (25%) d. Collagen binding assay (CBA): 4 (7%) It seems the collagen […]

Bleeding Disorders

TPA for Pleural Effusion

A colleague contact me on August 24 about a relative who is an inpatient and is experiencing a pleural effusion subsequent to a MRSA infection. The MRSA has been treated and is resolved, but the effusion is mucinous and drains slowly. The surgeon was planning to use recombinant tissue plasminogen activator (TPA, Alteplase) to liquify […]

Fibrinolysis

How Rapidly Does INR Decrease?

Here is a question that came to me from the American Society for Clinical Laboratory Science Consumer Web Forum: How quickly will an elevated international normalized ratio (INR, PT/INR) decrease/time? I appreciate the answer may be “it depends,” but for patients on warfarin and with an elevated INR (for example 4.0 in a patient with and INR […]

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Anticoagulant Therapy

Aug 23 2012

george

Mix Corrects with NP, But not with Deficient Plasmas

From “Japgi,” I have a 6 year old boy, well till a month back, when he had a post-traumatic gum bleed. The bleed was stopped after about half an hour, but a small ooze restarted and stayed for 3 days. His prothrombin time (PT) is 14 seconds (RI 11–16), activated partial thromboplastin time (APTT, PTT) […]

Lupus Anticoagulant

An Unexpected VWD by Lab Diagnosis

I learned about an interesting case at the University of Alabama at Birmingham(UAB) Hospital special coagulation lab today from specialty techs Patti Tichenor andLaura Taylor. A specimen arrived with an order for lupus anticoagulant profile and anti-cardiolipin antibody. The PTT was prolonged and corrected on initial mix. The UAB protocol recommends a two-hour incubation and repeat mixing study […]

Bleeding Disorders

Factor VIIa-AT Assay

Paul Riley, PhD, mentions Stago’s new research use only assay Asserachrom VIIa-ATthat measures the activated factor VIIa-antithrombin complex (VIIa-AT, similar to the familiar thrombin-antithrombin complex), an analyte elevated in hypercoagulable states. Dr. Riley suggests the assay may be a potential surrogate biomarker for activated coagulation states. The press release and a series of references are attached […]

Factor Assays

Dabigatran Calibrators?

From Crystal Azevedo: I am currently evaluating the ECA-T kit to monitor dabigatran therapy. Are you aware of any labs/companies that have a set of validation plasmas? Sorry, Crystal, but no one is currently distributing dabigatran calibrators or controls, including the product developers at Diagnostica Stago, who have developed the research use only ecarin clotting assay (ECA) for […]

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Anticoagulant Therapy

Aug 16 2012

george

A POC DTI Monitor

At the ASCLS/AACC meeting in July I had the opportunity to chat with Tonya Kiziah, MT (ASCP), Helena Laboratories, about Helena’s new hand-held Abrazo instrument, currently research use only, which offers a new point-of-care (POC) direct thrombin inhibitor (DTI) assay, DTM. DTM is a dry chemistry modification of the Ecarin clotting time that employs paramagnetic iron oxide particle technology, […]

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Anticoagulant Therapy

All Posts

PT/INR Results Using ISI 1.0 Reagents

New QQ: History

Quick Question Summary: VWF Activity

TPA for Pleural Effusion

How Rapidly Does INR Decrease?

Mix Corrects with NP, But not with Deficient Plasmas

An Unexpected VWD by Lab Diagnosis

Factor VIIa-AT Assay

Dabigatran Calibrators?

A POC DTI Monitor

George Fritsma MS MLS

Cheat Sheets from George

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