Has anyone been able to adapt a third-party reagent for ristocetin onto a Stago STAR-E or Compact? If so, would you be willing to share your experience with the product, validation procedure, etc? Please feel free to reply off-line if you so desire, and thank you in advance,
Dorothy Zdanowicz, Chemistry/Coagulation Supervisor
Englewood Hospital & Medical Center
Englewood, NJ 07631
Hello, Dorothy, and thank you for your question. I’ve known of one facility that successfully adapted the Siemens automated ristocetin cofactor assay reagent on a Stago STA several years ago, and another that was unable to use the reagent on the STAR-T. I’ll be interested in learning if any of our subscribers have made this work. Geo
From Dave McGlasson: Dorothy asked about the ristocetin co-factor (RCF) assay on the STA-R Evolution. I would suggest forget it and use the Beckman/IL LIA VWF assay. It worked great on our Evolution and have used it in a couple of protocols for publications. The RCF assay uses the Siemens formalinized platelets. We accomplished this procedure several years ago but it was cumbersome with CVs > 10%. The Beckman IL VWF LIA test had CVs < 5% on between-run and within-run testing for precision. It also had great sensitivity and specificity characteristics. Stago and Beckman/IL have test set-ups for the LIA method.