Mike Meyer asks, "we run three dilutions for all of our factor testing. Occasionally, we see results in which upon higher dilutions, the factor value decreases after adjusting for the dilution. For instance, I had a case today in which the FIX values were 0.64, 0.55, and 0.43 U/mL for the 1:10, 1:20, and 1:40 dilutions [values corrected for the dilution effect], the opposite effect seen with inhibitors. Calibration curve seems fine." He adds, "the patient’s FVIII level was very high [about 3.60 U/mL]. We possibly thought that the higher dilutions would bring the FVIII level back closer to a more normal range. The high concentration of FVIII in the first dilution may be accelerating the PTT clotting time yielding a falsely higher FIX value. Please comment."
Posts - Bleeding Disorders
Oct 23, 2020 12:44pm
Aug 6, 2020 7:47pm
A note from colleague Kathy Robinson that deserves a thorough discussion... "I am updating our mixing study procedure and have some questions..."
Aug 4, 2020 4:11pm
On August 4, 2020 the World Federation of Hemophilia announced the publication of the WFH Guidelines for the Management of Hemophilia, 3rd edition, 2020 [click for the publication].